Mutant of protease from extracting resisting to end in the corn of my scientist

Mutant of protease from extracting resisting to end in the corn of my scientist
Mutant of protease from extracting resisting to end in the corn of my scientist
The alternative wounds HIV and infects cells, offer the new thinking for the fact that HIV medicine is produced It is reported that passes the tackling of key scientific and technical problems for many years, recently, the intersection of roc and the intersection of column and the intersection of discipline and group and Chinese Academy of Sciences Shao, Professor of Chinese University of Hong Kong, the intersection of Kunming and the intersection of animal and Zheng, researcher of research institute, the intersection of Tang and discipline make up and make forever, obtaining one kind from the corn can wound HIV (HIV) selectively Infect the protease mutant of cells. This research results have offered new thinking and new strategy for researching and developing the specific target and infecting resisting HIV medicine to HIV newly of cells. It is reported, HIV hides mechanisms to hide and escape in cells the attack of host's immune system for a long time, what has already been listed has resisted HIV medicine and can not wound and infect cells and eliminate the virus selectively at present. Zheng Tang think new the intersection of research and thinking resist HIV medicine, seem very much important while being new developing forever, study, have, wound HIV, infect cell and protect normal cell from, resist AIDS injure medicine promising direction extremely selectively. Ribosomes inactivation albumen (RIPs) Have glucoside enzyme activity of RNAN - candy, can check the combination which lengthens the factor EF-1 or EF-2 and ribosomes, inhibit the biosynthesis of protein. So, RIPs has very high cell toxicity, are often developed and become immune toxin, antiviral or antineoplastic. RIP is divided into 3 kinds: I type, II type and III type. Among them, the Model III RIP does not have the active forebody albumen with structural land of internal inactivation of amino acid of a segment 25 to formate first represented by corn RIP, forebody's albumen just becomes active ribosomes inactivation albumen after excising in this structural land. The office, Ministry of Science and Technology in the Hong Kong subsidy for research " 973" Project, national great the intersection of science and technology and under the subsidy of event special, Chinese Academy of Sciences,etc., PengZhu, Zheng Shao Tang,etc. modify and transform a series of structure to the intersection of corn and internal the intersection of inactivation and structural land of RIP forever, have obtained corn RIP mutant that discerned and activated peculiar to HIV-1 protease. The research of the horizontal experiment of cell shows, the mutant, to not infecting low cell toxicity, but the mutant enters HIV-1 and can be discerned and cut and removed the structural land of inactivation to change into the active albumen by HIV-1 protease in cells behind infecting cells, thus wound HIV-1 and infect cells selectively. Meanwhile, enter efficiency of cells through increasing the mutant, it is stronger to infect the lethality of cells to HIV-1. The mutant can be able to bear HIV-1 protease too protease of one of medicine is discerned and activated, so mutant able to bear the intersection of medicine and, infect cell, have very fine choice antipersonnel too to the intersection of HIV-1 and protease. These research results have been in the international academic periodical " sour research of the core " (NucleicAcidsResearch) already Have and issue. (Zhang Wen Wen)


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